Armas, Pablo

Professional Career

 

– Bachelor in Biotechnology, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Año 2000.

Bachelor Thesis: “Caracterización de la expresión génica de una proteína de unión a ARNm durante el desarrollo embrionario en Anfibios”.

Director: Dr. Nora B. Calcaterra, PhD.

 

– PhD (Biological Sciences), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario. Año 2005.

PhD Thesis: “Caracterización molecular y funcional de la proteína celular de unión a ácidos nucleicos (CNBP) durante el desarrollo embrionario inicial”

Área Biología General, Departamento de Ciencias Biológicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario – IBR-CONICET.

Director: Dr. Nora B. Calcaterra, PhD.

 

– Post-doc. 2005 – 2007.

Topic: “Mecanismos genéticos y moleculares involucrados en la especificación y diferenciación durante el desarrollo embrionario de los vertebrados”.

IBR-CONICET.

Director: Dra. Nora Calcaterra.

 

– Investigador Asistente (Assistant Researcher) – CONICET. 2008 – 2012.

 

– Investigador Adjunto – CONICET. 2012 – 2017.

 

– Investigador Independiente (Independent Researcher) – CONICET. 2017 – present.

 

University Teaching:

– 2nd category Assistant. Área Biología General, Dto. Cs. Biológicas, Facultad de Ciencias Bioquímicas y Farmacéuticas (FCByF), Universidad Nacional de Rosario (UNR). 1996 – 1999.

 

– 1st category Assistant. Área Biología General, Dto. Cs. Biológicas, FCByF, UNR. 1999 – 2001.

 

– Head of practical work. Área Biología General, Dto. Cs. Biológicas, FCByF, UNR. 2001 – 2010.

 

– Assistant Professor. Área Biología General, Dto. Cs. Biológicas, FCByF, UNR. 2010 – present.

 

Other background:

 

– Award to the Ten Outstanding Young Persons in Santa Fe Province, TOYP SANTA FE 2016, Junior Chamber International (JCI) Rosario. Category: Scientific and Technological Development.

 

– Award to the Ten Outstanding Young Persons in Argentina, TOYP ARGENTINA 2016, Junior Chamber International (JCI) Rosario. Category: Scientific and Technological Development.

 

 

Research Lines

 

The research lines are focused on the study of the Guanine quadruplex nucleic acids secondary structure and their functions at different levels in the control of gene expression.

 

Gene expression regulation through Guanine quadruplex in embryonic development

Variations of gene expression levels by genetic polymorphisms in Guanine quadruplex

 

PhD Fellows

 

  • Ernesto J. Piga
  • Agustín Lorenzatti

 

 

Undergraduate Students

 

  • Georgina Bezzi

 

 

Selected Publications

  • David, A. P.; Pipier, A.; Pascutti, F.; Binolfi, A.; Weiner, A.M.J.; Challier, E.; Heckel, S.; Calsou, P.; Gomez, D.; Calcaterra, N.B.; Armas, P. (2019). “CNBP controls transcription by unfolding DNA G-quadruplex structures”. Nucleic Acids Res. pii: gkz527. DOI: 10.1093/nar/gkz527
  • Armas, P.; Calcaterra, N.B. (2018). “G-quadruplex in animal development: contribution to gene expression and genomic heterogeneity”. Mechanisms of Development. 154: 64-72. https://doi.org/10.1016/j.mod.2018.05.004
  • Armas, P.; David, A.P.; Calcaterra, N.B. (2017). “Transcriptional control by G-quadruplexes: in vivo roles and perspectives for specific intervention”. Transcription 8(1): 21-25. DOI: 10.1080/21541264.2016.1243505
  • David, A.P.; Margarit, E.; Domizi, P.; Banchio, C. E.; Armas, P.* (Co-corresponding author); Calcaterra, N.B.* (2016). “G-quadruplexes as novel cis-elements controlling transcription during embryonic development”. Nucleic Acids Res. 44(9): 4163–4173. DOI: 10.1093/nar/gkw011
  • Margarit, E.; Armas, P.; García Siburu, N.; Calcaterra, N.B. (2014). “CNBP modulates the transcription of Wnt signaling pathway components”. Biochim Biophys Acta 1839(11):1151-1160. DOI: 10.1016/j.bbagrm.2014.08.009
  • Bichara, D.; Calcaterra, N.B.; Arranz, S.E.; Armas, P.* (Co-corresponding author); Simonetta, S.H.* (2014). “Set-up of an infrared fast behavioral assay using zebrafish (Danio rerio) larvae, and its application for high-throughput compound biotoxicity screening”. J Appl Toxicol. 34: 214–219. DOI: 10.1002/jat.2856
  • Challier, E.; Lisa, M.N.; Nerli, B.B.; Calcaterra, N.B. and Armas, P. (corresponding author). (2014) “Novel high-performance purification protocol of recombinant CNBP suitable for biochemical and biophysical characterization”. Protein Expr. Purif. 93: 23–31. DOI: 10.1016/j.pep.2013.10.006
  • Armas, P.; Margarit, E.; Allende, M.L.; Calcaterra, N.B. (2013). “Beyond the binding site: in vivo identification of tbx2, smarca5 and wnt5b as molecular targets of CNBP during embryonic development”. Plos ONE. 8(5): e63234. DOI:10.1371/journal.pone.0063234
  • Borgognone, M.; Armas, P.; Calcaterra, N.B. (2010). “Cellular nucleic acid binding protein, a transcriptional enhancer of c-Myc, promotes the formation of parallel G-quadruplexes”. Biochem J. 428(3): 491-498. DOI:10.1042/BJ20100038
  • Armas, P.; Agüero, T.; Borgognone, M.; Aybar, M.J.; Calcaterra, N.B. (2008). “Dissecting CNBP, a zinc-finger protein required for neural crest development, in its structural and functional domains”. J Mol. Biol. 382: 1043–1056. DOI: 10.1016/j.jmb.2008.07.079
  • Armas, P.; Nasif, S.; Calcaterra, N.B. (2008). “Cellular Nucleic Acid Binding Protein binds G-rich single-stranded nucleic acids and may function as a nucleic acid chaperone”. J Cell. Biochem. 103: 1013-1036. DOI: 10.1002/jcb.21474

 

 

Collaborators

  • Miguel Allende; Departamento de Biología, Facultad de Ciencias, Universidad de Chile. Santiago, Chile.
  • Dennis Gomez-Zamorano. Institut de Pharmacologie et Biologie Structurale (IPBS) – CNRS. Toulouse, Francia.
  • Andrés Binolfi. IBR-CONICET, Rosario, Argentina.
  • Julia Cricco. IBR-CONICET, Rosario, Argentina.
  • Luján Álvarez. IFISE-CONICET, Rosario, Argentina.

 

 

Grants

  • ANPCYT – PICT 2017-0976. IR: Pablo Armas.
  • ASaCTeI Santa Fe. IA-2018-0075. Director: Nora Calcaterra.
  • ANPCYT – PICT 2016-0671. IR: Nora Calcaterra.
  • CONICET – PIP 2015-0170. IR: Nora Calcaterra.
Armas, Pablo

Position: Investigador Independiente
Laboratory: Bioquímica y Biología Molecular del Desarrollo
Sede: CCT
Email: armas@ibr-conicet.gov.ar
Tel. Interno: 654