Functional Genomics Plant-Pathogen


Research projects in our laboratory are broadly focused on different aspects of the molecular mechanisms of defence and pathogenesis of diseases induced by virus and bacteria in plants.

Research Lines

Functional genomics applied to the study of PVX and Xanthomonas pathogenesis in higher plants

1- Functional genomics applied to the study of PVX pathogenesis in Solanum tuberosum The goal of this project is to understand the biochemistry and genetic bases of resistance to potato virus X (PVX) mediated by the resistance gene Nb in S. tuberosum. Chromosome V of wild and cultivated potato contains a cluster of R-genes encoding resistance to diverse pathogens. The short arm of this chromosome harbors genes encoding resistance to Phytophtora infestans (R1) and hypersensitive resistance to the ROTH 1 strain of Potato virus X (Nb). Our research group as part of a joint effort with the NSF Potato Genome Project has constructed an integrated geneticphysical map of the Nb-R1 region using molecular markers from the tetraploid cultivar Pentland Ivory (genotype Nb, R1) and the allohexaploid wild species Solanum demissum. Sequence analysis of the region (~ 2 Mb) corresponding to the three haplotypes of Solanum demissum predicts the presence of more than 40 RGCs (resistance gene candidates) belonging to three distinct resistance-gene families (potato R1; tomato Prf and Bs4). Genetic and molecular analysis show that one of the RGCs sequences belongs to Nb. Our laboratory had also developed an agroinfiltration system to assess the transient expression of the viral elicitor, the 25 kDa protein, which will be used for functional complementation analysis of Nb-RGC. Towards the goal of elucidating defense signaling pathways, we have also studied the role of salicylic acid (SA) in Nb-mediated resistance to PVX infection, we have shown that SA is involved in the defense response mediated by Nb in Solanum tuberosum but the role played is notably different to that reported for the experimental model Arabidopsis thaliana and Nicotiana tabacum. 2- Functional genomics applied to the study of Xanthomonas pathogenesis in Citrus limon Xac is responsible for the canker disease affecting citrus plants throughout the world. The goal of this study is to generate knowledge for the development of crop protection methods based on interference with key biological and molecular processes in the epiphytic and disease phases of bacterial pathogenesis. We have studied the formation of biofilms of Xac and its role in the epiphytic survival and canker pathogenicity. We have shown that Xac develop biofilm on either abiotic or biotic surfaces and the presence of plant-associated biofilms is correlated with the bacterial survival and Xac pathogenicity. We have shown by genetic, biochemistry and molecular analysis that the biofilm formation of Xac requires xanthan production. Without xanthan, the bacteria are unable to retain water and cannot withstand abiotic stress, and thus cannot survive on the leaf surface. Based in our results we have established a Xac - Citrus limon assay and have used it to identify differential gene expression during Xac - Citrus interaction. In addition, we are developing genomic tools to study the function of these isolated genes in citrus.

Selected Publications

  • Roeschlin, R.A.; Favaro, M.A.; Chiesa, M.A; Alemano S.; Vojnov A.A.; Castagnaro A.P.; Filippone P., Gmitter F.G. Jr.; Gadea J. & Marano M.R. (2017). Resistance to citrus canker induced by a variant of Xanthomonas citri ssp. citri is associated with a hypersensitive cell death response involving autophagy-associated vacuolar processes. Molecular Plant Pathology, 18:1267-1281
  • Favaro, M.A.; Micheloud, N.G.; Roeschlin, R.A.; Chiesa, M.A.; Castagnaro, A.P.; Vojnov, A.A.; Gmitter Jr., F.G.; Gadea, J.; Rista, L.M; Gariglio, N.F. & Marano, M.R. (2014). Surface barriers of mandarin cv. 'Okitsu' leaves make a major contribution to canker disease resistance. Phytopathology, 104(9):970-976.
  • Vojnov, A. and Marano, M.R. (2015) Biofilm formation and virulence in bacterial plant pathogens. In: Virulence mechanisms of plant pathogenic bacteria. Edited by Nian Wang, Jeffery Jones, George Sundin, Frank White, Saskia Hogenhout, Caroline Roper, Leonardo De La Fuente, Jong Hyun Ham. The American Phytopathological Society Press, 2015. Chapter 2, pp.21-34.
  • Chiesa, M.A.; Siciliano, M.F.; Ornella, L.; Roeschlin, R.A., Favaro, M.A.; Pino Delgado, N.; Sendín, L.N.; Orce, I.G.; Ploper, L.D.; Vojnov, A.A.; Gadea, J.; Filippone, M.P.; Castagnaro, A.P. & Marano, M.R. (2013). Characterization of a variant of Xanthomonas citri ssp. citri which triggers a host-specific defense response. Phytopathology, 103:555-564.
  • Sendín L., Filippone M.P., Orce I.G., Rigano L., Peña L., Enrique R., Vojnov A.A., Marano M.R. and Castagnaro A.P. (2012) Transient expression of Capsicum chacoense Bs2 gene in Citrus limon as an approach for management of citrus canker disease. Plant Pathology. Plant Pathology vol. 61 p. 648 - 657.
  • Malamud F., Torres P.S., Roeschlin R., Rigano L.A., Enrique R., Bonomi H.R., Castagnaro A.P., Marano M.R., Vojnov A.A. (2011) Xanthomonas axonopodis pv. citri flagellum is required for mature biofilm and canker development. Microbiology, 157, 819-829. DOI 10.1099/mic.0.044255-0.
  • Enrique R., Siciliano F., Favaro M.A., Gerhardt N., Roeschlin R., Rigano L., Sendin L., Castagnaro A., Vojnov A. and Marano M.R. (2011) Novel demonstration of RNAi in citrus reveals importance of citrus callose synthase in defence against Xanthomonas citri subsp. citri. Plant Biotech. J. 9, 394-407.
  • Rigano L., Marano M.R., Castagnaro A.P., Do Amaral A.M. and Vojnov A.A. (2010) Rapid and sensitive detection of Citrus Bacterial Canker by loop-mediated isothermal amplification combined with simple visual evaluation methods. BMC Microbiology 10, 176.
  • Vojnov A.A., do Amaral A.M., Dow J.M., Castagnaro A.P., and Marano M.R. (2010) Bacteria causing important diseases of citrus utilize distinct modes of pathogenesis to attack a common host. Appl Microbiol Biotechnol, DOI 10.1007/s00253-010-2631-2.
  • Sánchez G., Gerhardt N., Siciliano F., Vojnov A.A., Malcuit I. and Marano M.R. (2010) Salicylic acid is involved in the Nb-mediated defense responses to PVX in Solanum tuberosum. Mol. Plant-Microbe Interact. 23, 394-405.



  • Vojnov, Adrián. Instituto de Ciencia y Tecnología Dr. Cesar Milstein-CONICET, Fundación Pablo Cassará, Ciudad de Buenos Aires.
  • Castagnaro, Atilio. Instituto de Tecnología Agroindustrial del Noroeste Argentino (ITA-NOA)-CONICET, Estación Experimental Agroindustrial Obispo Colombres (EEAOC), Tucumán.
  • Gariglio, Norberto. Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza (Sta. Fe).
  • Ing. Agr. Agostini, Juan P. INTA Montecarlos, Misiones.
  • Dra. Chiesa, Amalia  (FCA – UNR).
  • Dra. Favaro, Alejandra  (FCA – UNL).
  • Dra. Roeschlin, Roxana  (EEA - INTA Reconquista/ CONICET).


  • Dr. Gadea Vacas, José. Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia, Valencia, España.
  • Dr. Gmitter, Frederick G. Jr., Centro de Educación e Investigación de los cítricos (CREC), Universidad de Florida, Estados Unidos.
  • Dr. González, Claudio. Departamento de Microbiología y Cs. De la Célula, Instituto de Genética, Universidad de Florida, Estados Unidos.


  • Proyecto de Unidad Ejecutora – Gran Área de Ciencias Biológicas y Médicas – Convocatoria 2017 – CONICET. “Superando barreras en la producción vegetal mediante la modificación de múltiples rutas biológicas”. 2018 - 2023.
  • Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) PICT-2016-1222. “Caracterización de las bases genéticas de la resistencia a la cancrosis de los cítricos mediada por un efector TAL PthA4 truncado de una variante natural de Xanthomona citri subsp. citri.”. 2018 – 2021.
  • Proyecto de Vinculación Tecnológica y Desarrollo Productivo. Universidad Nacional de Rosario. “Biocontroladores naturales en la defensa contra patógenos vegetales”. 2017 - 2018.
  • Programa de Cooperación Bilateral CONICET-CSIC PCB II 2013. “Genómica comparativa de especies de Xanthomonas citri subespecie citri y caracterización de redes regulatorias de genes involucradas en los procesos de defensa a Xanthonomas en cítricos” (Titular: Dra. María Rosa Marano, Contraparte: Dr. José Gadea). 2016 -2019.
  • ANPCyT PICT-2013-0400. “Identificación y caracterización funcional de factores de virulencia de Candidatus L. asiaticus, agente causal del Huanglongbing de los cítricos, mediante aproximaciones en sistemas hospedadores heterólogos”. 2014 - 2017.
  • International Citrus Genome Consortium (ICGC): Providing tools to address HLB and other challenges. Florida Citrus Advanced Technology Programm - Control of Citrus Greening, Canker and Emerging Diseases of Citrus. Centro de Educación e Investigación de los cítricos (CREC), Universidad de Florida, Estados Unidos. Subcontract Number UF13089. 2013.
  • ANPCyT PICT-2011-1833. “Estrategias Biotecnológicas para el manejo de la Cancrosis Bacteriana de los Cítricos”. 2012 - 2015.
  • Fundación Nuevo Banco de Santa Fe, Proyecto a la Innovación Tecnológica: “Inducción de la respuesta de defensa a patógenos virales en Solanum tuberosum y otras especies de Solanáceas aplicando compuestos bioactivos vegetales”. 2011 -  2012.
  • International Citrus Genome Consortium (ICGC): Providing tools to address HLB and other challenges. Florida Citrus Advanced Technology Programm - Control of Citrus Greening, Canker and Emerging Diseases of Citrus. Centro de Educación e Investigación de los cítricos (CREC), Universidad de Florida, Estados Unidos. Subcontract Number UF1150. 2012
  • UNR. Subsidio Secretaría de Ciencia y Técnica. 2007 – 2016.
  • Programa de Cooperación Científico-Tecnológica entre MINCYT y MICINN (España) ES/09/08. “Genómica Funcional en Cítricos: Análisis de la respuesta frente a patógenos de gran incidencia en la citricultura de Argentina y España”. 2009.
  • ANPCyT PICT-2007-00469. “Utilización de la genómica funcional para estudio de la respuesta de Citrus limon a Xanthomonas axonopodis pv. citri”. 2008 – 2011.

Director de Grupo

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Marano, María Rosa
Core CCT
Phone: +54 341 4237070
Office Extension: 653
Laboratory Extension: 624